|
KMID : 1134820080370030373
|
|
Journal of the Korean Society of Food Science and Nutrition
2008 Volume.37 No. 3 p.373 ~ p.378
|
|
|
Optimal Culture Conditions for Production of Subtilisin-like Protease
|
|
Kang Sung-Il
Jang Young-Boo
Choi Gyeong-lim
Choi Byeong-Dae
Kong Jai-Yul
Choi Yeung-Joon
|
|
|
Abstract
|
|
|
The objective of this paper was to investigate optimal culture conditions for the production of an inhibitor
against subtilisin-like protease from Streptomyces thermocarboxydus (S. thermocarboxydus) C12 isolated from
sediments of Gwangyang coast. The optimal temperature and initial pH for the production of subtilisin-like
protease inhibitor were 40oC and pH 8.0, respectively. Inhibition activities were high for galactose, glucose
and fructose. The best carbon source and its concentration were galactose and 1.6% (w/v), respectively.
Inhibition activities were also high in medium containing polypeptone, proteose and peptone. Optimal nitrogen
source and concentration were protease peptone and 0.5% (w/v), respectively. Optimal concentrations for
inhibitor production were 1% (w/v) for NaCl and 1 mM LiCl for metal salts. The subtilisin-like protease
inhibitor from S. thermocarboxydus C12 showed a maximum inhibitor activity after cultivation for 84 h under
the optimized medium.
|
|
|
KEYWORD
|
|
|
Streptomyces thermocarboxydus, marine actinomycete, protease inhibitor, subtilisin, optimum conditions
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|